Supplementary Figure 3: Structure of the HD nuclease domain of PaCas3.
From: Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3
(a) Structure of the HD nuclease domain of PaCas3. Two calcium ions are shown as yellow spheres. The long loops L4, L5 and L6, which are bound to AcrF3, are labeled as indicated.
(b) The catalytic center of HD. Two calcium ions and one water molecule are shown as yellow and red spheres, respectively. The calcium-binding residues are shown as sticks and labeled as indicated.
(c) Structural comparison of the HD of PaCas3 with the HD nuclease domain of Cas3 from Thermobifida fusca (TfCas3). TfCas3 is shown in red. The protruding helix α6 with the long linking loops L5 and L6 are labeled as indicated.
