Supplementary Figure 7: Structural comparison of the PaCas3–AcrF3 complex with the TfCas3–DNA complex.
From: Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3
The HD domain, RecA1, RecA2, the Linker region and CTD are colored in cyan, grey, yellow, blue and red, respectively, for both TfCas3 and PaCas3. AcrF3 is represented as a surface and colored as in Figure 1b. The ssDNA in the TfCas3-DNA complex is shown in cartoon. The entrance of the DNA-binding tunnel formed by RecA2 and the CTD in TfCas3 are labeled as indicated (left panel). PaCas3 contains a similar DNA-binding tunnel (middle panel). AcrF3 binding blocks the DNA-binding tunnel in PaCas3 (right panel).
