Supplementary Figure 4: The large Hsc70-induced increase in scattering at pH 6.0 is not due to cage aggregation, because the amplitude and rate of the scattering increase is insensitive to cage concentration.
From: Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation
Hsc70 could induce large increases in scattering by causing cages to aggregate. Since aggregation would be at least a 2nd-order reaction, its rate should be sensitive to cage concentration. A. Scattering vs. time in pH 6.0 disassembly reactions with [Hsc70] at 2 μM and clathrin cages at the indicated concentrations (expressed in molar concentration of CHC with data plotted representing averages +s.e. of 10, 19, 15 and 14 replicates at 38, 75, 150 and 300 nM, respectively) and with scattering normalized so that the starting level in the reaction with 150 nM cages is 1.0. B. Data from A replotted so that the starting level of scattering in all the reactions is normalized to 1.0.
