Supplementary Figure 7: Hsc70, but not Hsc70ΔC, disassembles cages and forms filamentous oligomers at pH 6.0.
From: Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation
Cages were mixed with ATP and either Hsc70ΔC or Hsc70 as described for cryo-EM, but then incubated for 30 minutes to allow disassembly to occur and then negatively stained for EM. In the Hsc70ΔC reaction, cages were still present against a background of excess Hsc70ΔC (panel A). In reactions with Hsc70 almost no cages could be seen (panel B), though clearly obvious triskelia were not visible because the spindly triskelia are likely obscured by Hsc70 molecules in 10-fold molar excess of CHC. Long Hsc70 oligomers were abundant (a higher magnification view of these is shown in panel C), with each unit of the oligomer estimated as large enough to accommodate 1-2 Hsc70 molecules. In panels A and B a model of a reconstructed cage is inset to provide a scale marker.
