Supplementary Figure 9: Cryo-EM analysis of the human 26S proteasome bound to USP14–UbAl. | Nature Structural & Molecular Biology

Supplementary Figure 9: Cryo-EM analysis of the human 26S proteasome bound to USP14–UbAl.

From: An atomic structure of the human 26S proteasome

Supplementary Figure 9

(a) A flow-chart for the cryo-EM analysis of the human 26S proteasome bound to USP14-UbAl. 534,096 particles were subjected to 2D classification and subsequent auto-refinement. After 3D classification, 182,079 particles were picked for the following 3D refinement. With the soft mask on CP-RP2 and RP, reconstructions at average resolutions of 4.2 Å and 4.35 Å, respectively, were produced. Please refer to Materials and Methods for details. (b) Cryo-EM density map of the human 26S proteasome bound to USP14-UbAl calculated with the RP mask displays an average resolution of 4.35 Å. Two perpendicular views are shown. The resolution range is color-coded. Red circles indicate the location of USP14-UbAl. (c) FSC curves of the two cryo-EM reconstructions: human 26S proteasome with the CP-RP2 mask (cyan) and with the RP mask (purple).

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