Supplementary Figure 7: Additional hybrid live-cell SLB assay data and replotted overlays.
From: One-way membrane trafficking of SOS in receptor-triggered Ras activation
(a) The anti-BCR antibody was coupled to the bilayer via Cy5-labeled streptavidin thus providing a readout for the location of activated BCR microclusters whereas SOS was visualized via EGFP tagging.
(b) Representative microscopy images collected from live cell experiments for various SOS constructs. Bright field (top row), RICM (second row), EGFP (third row), and Cy5 (bottom row) channels of cells expressing different SOS-EGFP constructs (one cell for each column). The images were taken 20-40 minutes after cell landing and spread on the antibody-functionalized supported lipid bilayers. Information on number of samples for each construct is given in Figure 6b. Bar: 3 µm.
(c) Overlay micrographs from Figure 6c replotted as separate image channels. Bar: 3 µm.
(d) Overlay from the inset in Figure 7d replotted as separate image channels.
