Supplementary Figure 2: Proximity ligation assay (PLA) shows SNX27 interaction with GluN2B.
From: A molecular code for endosomal recycling of phosphorylated cargos by the SNX27–retromer complex
(related to Fig. 5)
Hippocampal neurons (DIV18) were transfected with GFP-SNX27 and stained with specific antibodies against GFP, GluN2B and MAP2 prior to PLA assay. The PLA signals between GFP-SNX27 and endogenous GluN2B are shown in the red channel, while GFP-SNX27 and MAP2 are labeled in green and blue channels, respectively. Scale bars = 50 μm (left), 20 μm (top right) and 5 μm (bottom right).
