Supplementary Figure 2: Amino acid sequence and RP-HPLC profile of fully oxidized Con-Ins G1. | Nature Structural & Molecular Biology

Supplementary Figure 2: Amino acid sequence and RP-HPLC profile of fully oxidized Con-Ins G1.

From: A minimized human insulin-receptor-binding motif revealed in a Conus geographus venom insulin

Supplementary Figure 2

The left-hand panel shows the amino acid sequence of fully-oxidized Con-Ins G1 (top: A chain; bottom, B chain). O: hydroxyproline, γ: γ-carboxyglutamate, *: amidated C-terminus. The right-hand panel shows the RP-HPLC profile. RP-HPLC conditions are: C18 Vydac RP-HPLC column, linear gradient ranging from 15 to 45% of solvent B in 30 min with 1 mL / min flow rate monitored at 220 nm.

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