Supplementary Figure 4: Influence of PI(4,5)P2 and salt concentrations on the binding of Sky1–353 to liposomes.
From: Skywalker-TBC1D24 has a lipid-binding pocket mutated in epilepsy and required for synaptic function
(a) Western blots of liposome flotation assays using wild type Sky1-353 and liposomes (PC:PS) enriched with different concentrations of PI(4,5)P2 (0%, 0.5%, 2% and 5%). n = 2 independent experiments.
(b) Western blots of liposome flotation assays using wild type Sky1-353 and liposomes (PC:PS) enriched with 2% of PI(4,5)P2 in the presence of three different NaCl concentrations (30 mM, 150 mM and 500 mM). n = 2 independent experiments.
Original blots can be found in Supplementary Data Set 1.
