Supplementary Figure 6: Cotranscriptional SHAPE-seq data for fluoride riboswitch mutants M18 and M22 with disrupted pseudoknots.
From: Cotranscriptional folding of a riboswitch at nucleotide resolution

(a) Reactivity matrices of the M18 mutant transcribed with either 10 mM (top) or 0 mM NaF (bottom). (b) Reactivity differences (∆ρ) between the matrices in (a). Positions that are red are more reactive when fluoride is present, while positions that are blue are more reactive without fluoride. (c) Single nucleotide trajectories (taken from (a)) displaying changes in the reactivities of key nucleotides, or nucleotides representing key regions, when transcribed with either 0 mM (gray) or 10 mM NaF (black). (d) As in (a), but for mutant M22. (e) Reactivity differences (∆ρ) between the matrices in (d). (f) Single nucleotide trajectories (taken from (d)) displaying changes in the reactivities of key nucleotides, or nucleotides representing key regions, when transcribed with either 0 mM (gray) or 10 mM NaF (black). Results shown are n=1.