Supplementary Figure 2: Cotranscriptional DMS probing data for SRP RNA.
From: Cotranscriptional folding of a riboswitch at nucleotide resolution
(a) SHAPE-seq reactivity matrix for SRP RNA (as shown in Fig. 2b, provided here for comparison with (b)). (b) SHAPE-seq reactivity matrix for all of the intermediate transcript lengths of the SRP RNA that were cotranscriptionally probed using DMS. Overall, similar transitions are observed with DMS, though the DMS data shows gaps in reactivity since it is selective for A and C bases. Reduced reactivity with DMS in the early hairpin loop (nts 9 – 19) is due to the presence of almost exclusively G and U bases in this region (Fig. 2a). Results shown are n=1 and are representative of three biological replicates (Supplementary Fig. 9b).
