Supplementary Figure 6: Putative sites of Cl– and Zn2+.
From: Crystal structures of human glycine receptor α3 bound to a novel class of analgesic potentiators
(a) The putative Cl- site with Cl- shown as green sphere and the side chains of Ala254 and Thr258 shown in sticks. The Fo-Fc omit map is contoured at 5σ. Subunit B has been removed for clarity. Distances from side chain carbons to Cl- are 5.4 – 6.4 Å. (b) The putative binding site of Zn2+ with Zn2+ shown as grey sphere and the side chains of Glu192, Asp194, and His215 shown as sticks. The NCS-averaged anomalous difference map is contoured at 5σ. Distances from Zn2+ to side chain oxygen atoms average 2.2 Å and to His215 nitrogen atoms average 2.6 Å across all five subunits.
