Supplementary Figure 7: Binding of glycine to GlyRα3cryst without and with bound AM-3607.
From: Crystal structures of human glycine receptor α3 bound to a novel class of analgesic potentiators
(a) Determination of glycine affinity to apo GlyRα3cryst measured by SPR. KD value is mean ± s.e.m., determined from N=2 experiments. (b) Determination of glcyine affinity to AM-3607 bound GlyRα3cryst by ITC. Orthosteric ligand glycine was titrated into purified GlyRα3cryst preincubated with AM-3607. A representative titration is shown with non-linear best fit. Binding parameters were determined from N=2 experiments and are means ± s.d..
