Supplementary Figure 3: CO modulates clock-gene expression at the transcriptional level.
From: Reciprocal regulation of carbon monoxide metabolism and the circadian clock
(a) Clock gene mRNA and pre-mRNA levels of primary fibroblasts of Ho-1-/- mice (KO) or wild-type littermates (WT) harvested 24 hours after dexamethasone synchronization. Data are normalized to Gapdh expression and presented relative to mean expression in wild-type control cells. Given is mean ± sd of three independent samples. (b) Pre-mRNA levels of Dbp in embryonic fibroblasts from Ho-1-/- mice (KO) or wild-type littermates (WT) 24 hours after dexamethasone synchronization, which were treated for 1 hour with 100 μM CO-releasing molecules (CORM) or inactive control molecules (iCORM) before harvesting. Data are normalized to Gapdh expression and presented relative to mean expression in wild-type control cells. Given is mean ± sd of three independent samples. (c) CO does not acutely alter BMAL1 protein level. BMAL1 protein levels in U2-OS cells 24 hours after dexamethasone synchronization, which were treated for 1 hour with 100 μM CO-releasing molecules (CORM) or inactive control molecules (iCORM) before harvesting. Shown are four independent samples.
