Supplementary Figure 3: Unmodeled electron density peaks in the portal and central cavity.
From: Crystal structure of the MOP flippase MurJ in an inward-facing conformation
(a) The protein is sectioned on the dotted plane and viewed from the periplasm (along the direction of the arrow) to visualize the portal. The non-protein 2Fo−Fc electron density peaks are shown as magenta mesh, contoured to 0.7 σ with the protein electron density masked. Gray sticks denote monoolein molecules. (b) The protein is sectioned and viewed from the right to visualize the distal site of the central cavity. The 2Fo−Fc electron density peaks are contoured to 0.7 σ and carved 2.0 Å from the pentapeptide model. (c) Model of the pentapeptide (L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala) built into the density in panel b, but which was not used for refinement.
