Supplementary Figure 1: Microscopy data analysis and validation.
From: Self-correcting mismatches during high-fidelity DNA replication
(a) Representative micrograph of the PolIIIα-clamp-exonuclease-θ-τ500-DNA complex. (b) 2D class averages derived from the initial 148,532-particle dataset. (c) Schematic representation of the cryo-EM data processing strategy. (d) Fourier shell correlation for the PolIIIα-clamp-exonuclease-θ-DNA complex with and without focussed classification. The correlation between two independently refined halves of the data is indicated in solid lines (gold-standard FSC) and the correlation between the rigid-body docked model and the map in dashed line. (e) Local resolution map for the whole reconstruction and the map after focussed classification and alignment of clamp-signal subtracted particles. See also Supplementary Video 3. (f) Example of the PolIIIα subunit fitted into the cryo-EM map. (g) Examples of the DNA fitted into the cryo-EM map.
