Supplementary Figure 3: DNA polymerization and editing modes of A-, B-, and C-family DNA polymerases.
From: Self-correcting mismatches during high-fidelity DNA replication
(a) Polymerization and (b) editing modes of A, B, and C-family DNA polymerases are shown in cartoon representation. Polymerase domains are colored in orange and exonuclease domains in yellow. Structures for polymerization mode are: T. aquaticus Pol I (1QTM (Li, Y. et al., Proc. Natl. Acad. Sci. USA 96, 9491–9496, 1999)), E. coli Pol II (3K57 (Wang, F. et al., Cell 139, 1279–1289, 2009)), E. coli PolIIIα (5KFW (Fernandez-Leiro R. et al., Elife 4, 459, 2015)). Structure for the editing mode are: E. coli Pol I (1KLN)(Beese, L. S. et al., Science 260, 352–355, 1993)), P. abyssi Pol B (4FLW (Gouge, J., et al., J. Mol. Biol. 423, 315–336, 2012)), E. coli PolIIIα (this work) (c) Comparison of the DNA molecules in both modes. Light gray: polymerase mode, black: editing mode. (d) Close-up of exonuclease active sites of Pol I, Pol B, and Pol III. Catalytic residues are shown in stick representation and colored green. D357 in Pol I (shown in white sticks) was mutated to alanine in the structure and was mutated back to the original aspartate for clarity.
