Supplementary Figure 4: Model building strategies and cryo-EM densities at different regions. | Nature Structural & Molecular Biology

Supplementary Figure 4: Model building strategies and cryo-EM densities at different regions.

From: Structural insights into the secretin translocation channel in the type II secretion system

Supplementary Figure 4

(a, b) Model building strategies for different regions of K12_GspD (a) and Vc_GspD (b). Atomic models of N3 domain, secretin domain and S domain (cyan) were generated by de novo building and atomic models for N1 domain and N2 domain (magenta) were based on homology model building. (c-f) The representative cryoEM densities at different regions. Shown here were the cryoEM densities for N3 domain (c), S domain (d), outer barrel subdomain (e) and inner barrel subdomain (f). The figures were prepared using PyMol at the 6σ contour level of the density maps.

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