Supplementary Figure 2: Cryo-EM images and 2D classification analysis.
From: Structural insights into the secretin translocation channel in the type II secretion system
(a,b) Representative cryoEM raw images of K12_GspD (a) and Vc_GspD (b). Top view and side view particles were shown in blue and red cycles, respectively. (c, d) 2D class averages of K12_GspD particles (c) and Vc_GspD particles (d). Side views (above) and top views (below) showed clear secondary structural features. Red arrows pointed to smeared densities, indicating flexibility of N0 domain. (e) Side views of K12_GspD, which exhibited slightly tilted N1-N2 domains (left) compared with other class averages (right).
