Supplementary Figure 3: Local resolutions and Fourier shell correlation (FSC) curves.
From: Structural insights into the secretin translocation channel in the type II secretion system
(a, b) Local resolution cryoEM density maps for K12_GspD (a) and Vc_GspD (b) colored according to resolutions estimated by ResMap. The outer (left) and inner (right) surfaces are displayed for each map. Unstructured densities of cap gate, central gate and N3 constriction site were emphasized with white, red and yellow ellipses, respectively. (c, d) Gold standard FSC curves (cyan) of whole K12_GspD channel (c) and Vc_GspD channel (d). FSC curves between the atomic model and the cryoEM density map are shown in red. (e, f) Cross-validations for K12_GspD (e) and Vc_GspD (f). Whole model versus summed map (black), whole model versus half map 1 (red, work) and whole model versus half map 2 (cyan, free). See Methods for details.
