Supplementary Figure 4: Local resolution and FSC curves of the 4.2-Å structure (PC2MI) and 4.3-Å structure (PC2SI).
From: Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2
Local map resolution (rainbow bar, in Å) was determined with RESMAP. (a) Exoplasmic, (b) side and (c) cytoplasmic view of PC2MI and respective views (h-g) of PC2SI. (d) Histogram of the local resolution indicates a mean resolution of ~4.0 Å for PC2MI and 4.0 - 4.5 Å PC2SI. (e) Correlation of the masked and unmasked map with the Phenix-refined model for the 4.2 Å and (k) for the 4.3 Å dataset. (f) Correlation of individual half-maps (blue and magenta) and summed map of the the 4.2 Å structure refined with Phenix (red) or REFMAC (green), compared to the respective correlation of the 4.3 Å structure (l).
