Supplementary Figure 1: PC2 expression in HEK293 GnTI– cells.
From: Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2
(a) Laser confocal microscopy after 48 h of PC2 expression. (b) Western blot analysis against the C‑terminal domain of PC2 of Triton-solubilized HEK 293 GnT I- cells (1) before induction, (2) 6 h, (3) 12 h, (4) 24 h, (5) 48 h, and (6) 72 h after induction. (c) Crystalloid formation in HEK 293 GnT I- cells in thin plastic sections overexpressing PC2 12 h to 72 h after induction. (d) Ion ablation tomography and 3D reconstruction reveals that crystalloids after 72 h are a honeycomb of tubular ER vesicles. (e) Immuno-gold labeling of the PC2 C‑terminal domain (C-term) in thin plastic sections of whole cells after 48 h. Gold labels are visible as small black dots.
