Supplementary Figure 2: Purification and single-particle cryo-EM of human PC2.
From: Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2
(a) Gel filtration profile (Superose-6) of PC2 in amphipol A8-35 after purification on streptactin resin. The PC2 tetramer elutes at a retention volume of about 1.35 ml. Absorption was measured at 280 nm. (b) SDS-PAGE of amphipol reconstituted PC2. (c) Cryo-micrograph of PC2 tetramers recorded at a defocus of 2.5 μm. (d) Four representative 2D class averages.
