Supplementary Figure 3: Single-particle processing.
From: Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2
267 010 particles were semi-automatically selected and subjected to 3D classification without symmetry imposed. The best class containing 162 074 particles was further classified with C4 symmetry, resulting in two good classes. Particles of both classes were combined and subjected to a 3D refinement, followed by particle polishing. Two distinct PC2 conformations were obtained by further 3D classification with C4 symmetry, followed by two independent 3D refinements of the two best 3D class averages.
