Supplementary Figure 2: Replicates and validation of C. elegans replication origins.
From: The gastrula transition reorganizes replication-origin selection in Caenorhabditis elegans
(a) Correlation of the nascent strand microarray data in two of the three replicates for the pre-gastrula and mixed embryo populations. (b) Heatmap representing a significant set of probes of the three replicates (R1-R3) of nascent strand microarray data for pre-gastrula (left) and mixed embryo (right) origins. Colors indicate the intensity of the microarray probe, from low (green) to red (high). (c) Alignment of three independent nascent strand microarray experiments (R1-R3) along C. elegans chromosome 1. The bottom panels show representative profiles of origins in the chromosome arms and center. The background signal from the L1-arrested larval sample was subtracted from each replicate (-L1). (d) Quantitative PCR validation of C. elegans replication origins detected by microarray analysis of the NS purification (n=3), together with the corresponding controls. The graph shows the microarray normalized log2-ratio data (samples compared with genomic DNA) as well as the position of inverted repeats.
