Supplementary Figure 5: Telomere lengths in yeast mutants and DNA-binding activities of various HsMRN complexes. | Nature Structural & Molecular Biology

Supplementary Figure 5: Telomere lengths in yeast mutants and DNA-binding activities of various HsMRN complexes.

From: Eukaryotic Rad50 functions as a rod-shaped dimer

Supplementary Figure 5

(a) rad50 mutants are defective in telomere maintenance. Telomere length after 0, 15 and 30 generations of growth (0, 1 and 2 days) at 30°C. Heterozygote diploids (RAD50/rad50hook) were included as 0 generations of growth. Genomic DNA was digested with PstI and telomeres were visualized by Southern blot using a telomere specific probe. (b-e) DNA binding activities of various HsMRN complexes. EMSA in the presence of AMPPNP with 0 (lane 1), 100 (lane 2), 200 (lane 3), 400 (lane 4), 600 (lane 5) and 800 nM of WT MRN (lane 6). (b)The WT MRN complex (800 nM) was incubated without AMPPNP in lane 7, (c) MRN with Rad50ΔCC, (d) MRN with Rad50 Pfhook mutant, (e) MRN with Rad50ΔCC-48.

Back to article page