Supplementary Figure 6: Nucleotide densities found at the Mcm4-Mcm7, Mcm6-Mcm4, Mcm7-Mcm3, Mcm2-Mcm6, Orc1-Orc4, Orc4-Orc5, Orc5-Orc3, and Cdc6-Orc1 interfaces.
From: Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1
Protein structures are shown in cartoon, ATPγS in sticks, and electron densities of the nucleotides are shown in gray meshes. The nucleotide density at the Orc4-Orc5 interface was the weakest among the eight sites. The Orc5-Orc3 interface was the smallest among the five Orc1-5 subunits, such that the complex could be divided into two sub-complexes of Orc1-Orc4-Orc5 and Orc3-Orc2. However, there was contact between Orc5 and Orc3 at the nucleotide-binding region such that the ATPγS density at the interface was clear.
