Supplementary Figure 9: Protein-DNA interaction and sequence alignment of Mcm2–7 at the DNA-binding regions.
From: Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1
(a) Detailed interaction between the protein subunits and the modeled 39-bp DNA. Left panel shows the first 20-bp DNA located in the top ORC-Cdc6 region. ORC-Cdc6 interacts with both strands. Right panel shows the remaining 19-bp DNA. The PS1 and/or H2I loops of Mcm2, Mcm4, Mcm6, and Mcm7 extensively interact with the last seven bases of the right strand. (b) Multi-sequence alignment of Mcm2-7. Regions underscored by green lines are the DNA-binding H2I and PS1 hairpins. Amino acids in blue directly contact DNA. The DNA-binding KA motif in the PS1 hairpin is highly conserved. The Mcm2, 4, 6, and 7 are grouped together to show the clustering of the DNA binding sites.
