Supplementary Figure 1: Cryo-EM image processing and 3D-reconstruction procedure.
From: Structural basis of Mcm2–7 replicative helicase loading by ORC–Cdc6 and Cdt1
Motion-corrected raw particle images were sorted first by 2D classification. After removing raw particles that did not produce “good” averages, about 600,000 particles remained. These particles were further sorted into six 3D classes. Finally, particles that produced the best two 3D classes were combined, yielding ~300,000 particles for final 3D refinement. The final 3D map had an estimated resolution of 3.9 Å.
