Supplementary Figure 6: Structure-based multiple sequence alignment of the SPD.
From: Cryo-EM structure of a metazoan separase–securin complex at near-atomic resolution
Secondary structure elements of CeSPD and CtSPD are shown above and below the alignment, respectively. Residues conserved in all aligned sequences are highlighted in red. Residues binding to the securin pseudo-substrate motif P sites P1 to P6 are boxed in wheat, yellow-green, light green, green, cyan and light blue. Catalytic site residues are marked with a red asterisk. The species abbreviations are: Ce (Caenorhabditis elegans), Hs (Homo sapiens), Mm (Mus musculus), Dr (Danio rerio), Sc (Saccharomyces cerevisiae), Ct (Chaetomium thermophilum).
