Supplementary Figure 7: Multiple sequence alignment of securin and separase substrates.
From: Cryo-EM structure of a metazoan separase–securin complex at near-atomic resolution
Sequence alignment of the separase-binding motif (SBM) of securin. Secondary structure elements are shown above the alignment. Securin pseudo-substrate motif sites P1 to P6 are indicated. Invariant residues are boxed in dark red, conserved residues in light red. b, Sequence alignment of cleavage sites of separase substrates (Scc1, Rec8, kendrin and Slk19) and the securin pseudo-substrate motif. Sites P1 to P6 are indicated. Separase cleaves substrates immediately C-terminal to an Arg residue at P1 (labeled).
