Supplementary Figure 1: Preparations and EM images of the C. elegans (Ce) separase–securin complex.
From: Cryo-EM structure of a metazoan separase–securin complex at near-atomic resolution
a, SDS PAGE gel of Ce separase-securin. b, Size exclusion chromatograph of purified complex. C, Typical cryo-electon micrographs of Ce separase-securin in vitreous ice (representative of 234 micrographs) (left) and on graphene oxide grids (representative of 2,559 micrographs) (right). d, Gallery of two-dimensional class averages of Ce separase-securin showing typical views representative of 100 two-dimensional classes collected in vitreous ice on holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The white scale bar corresponds to a distance of 100 Å. e, Gallery of two-dimensional class averages of Ce separase-securin showing different views representative of 100 two-dimensional classes collected on graphene oxide coated grids. The white scale bar corresponds to a distance of 100 Å. f, Gallery of two-dimensional class averages of 2D-class averages of human separase-securin showing different views representative of 100 two-dimensional classes collected on graphene oxide coated grids (Quantifoil Au R1.2/1.3, 300 mesh). The white scale bar corresponds to a distance of 100 Å. g, Angular distribution plot of C. elegans data sets collected in vitreous ice (left) compared to the angular distribution plot of the data used for the final reconstruction (right) is shown.
