Supplementary Figure 2: Resolution estimation and example of de novo model building.
From: Cryo-EM structure of a metazoan separase–securin complex at near-atomic resolution
a, Representative EM density for regions of separase with fitted coordinates. b, Gold standard Fourier Shell Correlation (FSC) curve and FSC curve between cryo-EM map and final atomic model of the Ce separase-securin complex. c, Cross-validation of model refinement by half maps. Shown are FSC curves between the atomic model and the half map (maphalf1) it was refined against and FSC curve between the atomic model and the other half map (maphalf2) that was not used during refinement. d, EM density map colour-coded according to local resolution. e, B-factor plot: Cartoon representation of C. elegans separase-securin complex with main-chain atoms colour-coded according to B-factors from low (blue) to high (red).
