Supplementary Figure 4: NTPase stimulation of ABCE1 by 40S subunit.
From: Structure of the 40S–ABCE1 post-splitting complex in ribosome recycling and translation initiation
a, Hydrolyzed γ-32P-GTP resulted in released 32Pi that was separated by thin layer chromatography and quantified by autoradiography. b, Time traces of 32Pi formation were normalized to GTP only and time point 0 min and analyzed by a linear fit. NTP hydrolysis by ABCE1 was stimulated by addition of 40S subunits. c, Corrected GTPase activity of ABCE1 only shows stimulation by 3-fold upon addition of 40S subunit. Data derived from the slope in panel b are given as mean ± s.e.m. (n = 5).
