Supplementary Figure 5: Dynamic conformation of the cantilever hinge.
From: Structure of the 40S–ABCE1 post-splitting complex in ribosome recycling and translation initiation
In the pre-splitting state (left, 3JAH)13, the cantilever hinge (CL) forms an α-helix, which is unwound in the ABCE1 post-splitting state (right). Isolated densities are low-pass filtered at 3.9 Å (contoured at 5 σ) and shown with the respective models. b, In the post-splitting state, the FeS cluster domain is mainly stabilized by two interactions of the cantilever arm: the backbone of Asn78 interacts with Tyr301 of NBD1. Density of the ABCE1 post-splitting complex was low-pass filtered at 3.9 Å (contoured at 5 σ).
13 Brown, A., Shao, S., Murray, J., Hegde, R.S. & Ramakrishnan, V. Structural basis for stop codon recognition in eukaryotes. Nature 524, 493-496 (2015).
