Supplementary Figure 5: Sequence alignment of LptG homologs and residues selected for functional analysis in the structure.

a. Sequence alignment of LptG homologs from five representative Gram-negative bacterial strains. b. Conserved hydrophobic and positive residues of LptG lining the “V”-shaped cavity selected for mutational studies. Conservation of LptG residues in different Gram-negative homologs is shown in ENDscript. Secondary structures are numbered within the respective domains. Conserved residues lining the inner surface of the “V”-shaped cavity were selected for mutational analyses are highlighted. The labeled residue types and numbers in both alignments correspond to those in E. coli.