Supplementary Figure 4: Sequence alignment of LptF homologs and residues selected for functional analysis in the structure.

a. Sequence alignment of LptF homologs from five representative Gram-negative bacterial strains. b. Conserved hydrophobic and positive residues of LptF lining the “V”-shaped cavity selected for mutational studies. Conservation of LptF residues in different Gram-negative homologs is shown in ENDscript. Secondary structures are numbered within the respective domains. Conserved residues lining the inner surface of the “V”-shaped cavity were selected for mutational analyses are highlighted. The labeled residue types and numbers in both alignments correspond to those in E. coli.