Supplementary Figure 5: MDM2 mutants can limit induction of p53 target genes.
From: Structural analysis of MDM2 RING separates degradation from regulation of p53 transcription activity
qPCR showing mRNA expression of p53 target genes (GADD45β is not a p53 target gene) is attenuated by MDM2 I440K, I440E but not by C464A. n = 3 independent experiments each. P values (one-way ANOVA with Tukey's post hoc test): v.s. EV No doxy (vertical), v.s. C464A No doxy (horizontal). “n.s.” indicates not significant vs. EV No doxy. F statistics and degrees of freedom of ANOVAs are reported in Supplementary Table 3.
