Supplementary Figure 1: Api137-induced ribosome stalling at the end of the ORFs
From: An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome
Toeprinting analysis of translation arrest in the synthetic ORF RST2 (left) and the natural ORF ermBL (right) mediated by PrAMPs. The toeprint bands corresponding to the ribosomes arrested by Api137 or the natural apidaecin 1a (Api1a) at the stop codon of the ORF are indicated with orange arrowheads; the bands representing the ribosome arrested by Onc112 at the start codon are marked with blue arrowheads. Sequencing lanes are shown. The nucleotides corresponding to the toeprint bands are indicated in the gene sequence on the side of the gels; orange brackets indicate codons positioned in the P- and A- sites of the Api-stalled ribosome; blue brackets indicate codons in the P- and A- sites of the Onc112-stalled ribosome. The gels are representatives of two independent experiments.
