Supplementary Figure 3: Binding of phage displaying Pep2-8 (TVFTSWEEYLDWVGSG) or Pep2-8V2A (TAFTSWEEYLDWVGSG) to PCSK9.
From: Discovery of a cryptic peptide-binding site on PCSK9 and design of antagonists
The single mutation of valine2 to an alanine reduced binding affinity to PCSK9. The reduced but still detectable binding of Pep2-8V2A to PCSK9 was suitable for its use as an anchor peptide to C-terminally attach an extension peptide library via the GSG linker.
