Supplementary Figure 4: miRNA profiling by small RNA-seq and validation by RT-qPCR.
From: NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing
(a,b) Counts of reference sequences (tRNAs, snoRNAs, spike-in RNA, rRNAs) between duplicated small RNA-seq libraries from siGFP-treated cells (a) or between siPSF and siGFP-treated cells (b). (c,d) Correlation of miRNA counts between duplicated libraries from siGFP-treated (c) or siPSF-treated HeLa cells (d). (e) A panel of miRNAs validated by RT-qPCR. (f) Heatmap representation of validated miRNA expression from the miR-17-92a locus (left) or other pri-miRNAs (right) in response to knockdown of various paraspeckle-associated proteins or NEAT1 as indicated. Data in e are presented as mean ± SEM (n=3, technical replicates). **P < 0.01; ***P < 0.001; NS, not significant, determined by two-tailed Student’s t test. Data source data are reported in Source Data for Supplementary Figure 4.
