Supplementary Figure 5: CLIP-seq analysis of PSF/NONO interactions with RNA.
From: NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing
(a) The anti-PSF immunoprecipitated complex trimmed with two different concentrations of MNase (1: 1,000 or 1: 50,000 dilution) and analyzed by autoradiography (bottom panel) and Western blotting (upper panel). (b) Reproducibility between independently constructed CLIP-seq libraries for NONO and PSF. Global comparison was performed with 1 Kb-binned genome. (c) The genomic distribution of NONO and PSF CLIP-seq peaks. (d) Additional examples of NONO and PSF binding on a set of pri-miRNAs in HeLa cell. Uncropped images of Western blots and autoradiography in a are shown in Supplementary Data Set 1.
