Supplementary Figure 6: Exogenous DGCR8 interacts with endogenous NONO.
From: NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing
(a) The pri-miRNA-based reporter assay for pri-miR-612 processing relative to pri-miR-17-92a processing in response to knockdown of the Microprocessor. (b) Detection of FLAG-tagged DGCR8 in anti-NONO immunoprecipitant. (c) Detection of NONO in FLAG-tagged DGCR8 immunoprecipitant. * indicates IgG heavy chain. Data are shown in a as mean ± SEM (n=3, cell culture). *P < 0.05; ***P < 0.001; NS, not significant, determined by two-tailed Student’s t test. Data source are reported in Source Data for Supplementary Figure 6. Uncropped images of Western blots in b and c are shown in Supplementary Data Set 1.
