Supplementary Figure 3: Schematic representation of contacts between LbaCas13a and crRNA.
From: Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme
a, The contact map illustrates only those contacts that have unambiguous electron density. Nucleotides that make up the 5′ handle and 3′ spacer are labelled as orange or blue, respectively. Planar stacking interactions are indicated by dashed lines, and hydrogen bonds/electrostatic interactions are indicated by arrows. Amino acids that are conserved within the A-cleaving subfamily or across the entire Cas13a family are indicated with one or two asterisks, respectively. b-c, Conserved LbaCas13a amino acid contacts to the sugar-phosphate backbone of spacer nucleotides A(6) and A(8).
