Supplementary Figure 5: K6R mutation of Ub^dist in Lys6-Ub₂.

(a) Positions of all lysine residues of Ub^dist in the zUSP30–Lys6-Ub₂ structure. The lysine and N-terminal methionine residues of Ub^dist are shown as ball-and-stick models. Lys6-Ub₂ and zUSP30 are shown as cartoon and surface models, respectively. The coloring scheme is the same as that in Fig. 2. (b) Close-up view of the area around Lys6* of Ub^dist (*; replaced by Arg in the present structure). The drawing scheme is the same as that in Fig. 2. Water molecules are shown as green spheres. (c) Fluorescence anisotropy-based kinetic analysis of zUSP30 for the wild-type or K6R^dist Lys6-Ub₂. The drawing scheme is the same as that in Supplementary Fig. 3. (d) Fluorescence anisotropy-based affinity measurement of zUSP30 (C73A) for the wild-type or K6R^dist Lys6-Ub₂. The drawing scheme is the same as that in Supplementary Fig. 2.