Supplementary Figure 3: Overall and local resolution estimation of all obtained cryo-EM maps.

Overall and local resolution estimation of (a) the overall map at 3.8 Å (overall map 1), (b) the core focused map at 3.6 Å (core map), (c) the overall map with focus on the head region at 4.1 Å (overall map 2), (d) 3’ domain and UtpA focused map at 4.1 Å (3’ domain map) and (e) the central domain focused map at 7.2 Å (central domain map). a-e The left panel shows Fourier Shell Correlation (FSC) curves for the unmasked (dashed black line), phase-randomized (solid grey line), the masked (dashed grey line) and the corrected map (solid black line). FSC curves calculated between the final model and map (light-blue), model2 and half map 1 (FSC test; red) or half map 2 (FSC work; yellow dashed line) are shown. An FSC value of 0.143 is indicated by a thin black line. Three views related by a 120-degree rotation of the obtained cryo-EM map are shown colored according to local resolution. The fourth density panel shows a slab view visualizing the resolution in the center. Local resolution was calculated using Resmap⁴³.