Supplementary Figure 1: C-terminal domains of CAMSAP3 do not confer MT minus-end specificity.
From: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins
(a) Schematic of the CAMSAP3 domain organization and the constructs used.
(b,c) TIRFM images and quantification of the binding of GFP-CAMSAP3 fragments to GMPCPP-stabilized MTs and dynamic GDP-MTs; values for GFP- CAMSAP1-D2 quantified in the same way are shown for comparison. The intensity is normalized to the average intensity of MBD on GMPCPP MTs. Scale bar, 2 μm.
(d) Sequence alignment of the linker region located upstream of the third coiled-coil in vertebrate CAMSAP1. Negatively and positively charged residues are highlighted in purple and cyan, respectively.
See also Supplementary Table 2.
