Supplementary Figure 2: The CKK is a highly conserved MT minus-end-tracking domain.

(a) The presence of CKK in extant eukaryotic lineages and its evolutionary history since LECA. The CKK domain can be found in four out of five eukaryotic supergroups: Eukaryotic supergroups are indicated by colored branches (Opisthokonta: purple, Amoebozoa: blue, Stramenopila-Alveolata-Rhizaria (SAR): red, Archaeplastida: green, Excavata: brown). Species tree depicting all species comprising our eukaryotic proteome database and the presences of the CKK domain as well as its inferred losses. The presence of CKK in LECA and indicated subsequent loss events were inferred according to Dollo parsimony, which allows for only a single invention. Asterisk: sequence derived from NCBI. Note that in the oomycetes, the loss of CKK domain seems relatively recent, because Phytophthora infestans and Albugo laibachii contain CKK, but downy mildew Hyaloperonospora parasitica lacks it.

(b) Multiple sequence alignment of the CKK domain in a subset of the species in (a). The supergroups to which the species encoding these sequences belong are indicated by the name colors as in (a). The numbering of amino acids is based on human CAMSAP1 sequence. The colored asterisks indicate the amino acids, which, when mutated to alanine, alter the CAMSAP1_mini signal at MT minus ends compared to wild type protein; red asterisk, signal reduced to less than 25%; black asterisk, signal reduced to 25-75%; cyan asterisk, signal increased above 100%.