Supplementary Figure 3: Measurement of membrane binding of CD28CD by the aromatic fluorescence emission (AFE) assay.
From: Dynamic regulation of CD28 conformation and signaling by charged lipids and ions
(a) Incubation of 2 μM CD28CD with 0.2 mM acidic POPG LUVs led to significant increase of AFE value but such an increase was not observed in that with 0.2 mM zwitterionic POPC LUVs.
(b-c) Titration of acidic POPG LUVs with the indicated concentrations into 2 μM CD28CD sample led to the gradient increase of AFE value (at 310nm).
For each condition, three independent samples were measured in one experiment. Data are representative of three independent experiments. Unpaired t-test was used for comparing each two groups. One-Way ANOVA was used to test whether POPG treatment could cause significant change of the AFE value, P < 0.0001 (C).
The center value and error bar in c denote mean and s.e.m.. Source data are available in Source Data 5.
