Supplementary Figure 3: Kinetic analysis of synthetic LLO substrates.

Normalized PglB activities as a function of varying concentrations of three LLO analogs (structures shown in Fig. 1a). Activities were normalized to the maximal turnover rate for each LLO analog independently to allow the comparison of the Michaelis constants KM. Each data point represents three independent cell cultures, calculated from the slope of the linear regression fit (error bars indicate s.d., n≥4)